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anti ha 3f10 hrp  (Roche)


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    Roche anti ha 3f10 hrp
    An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and <t>anti-HA</t> antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.
    Anti Ha 3f10 Hrp, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ha 3f10 hrp/product/Roche
    Average 86 stars, based on 1 article reviews
    anti ha 3f10 hrp - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "The NRC0 gene cluster of sensor and helper NLR immune receptors is functionally conserved across asterid plants"

    Article Title: The NRC0 gene cluster of sensor and helper NLR immune receptors is functionally conserved across asterid plants

    Journal: The Plant Cell

    doi: 10.1093/plcell/koae154

    An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and anti-HA antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.
    Figure Legend Snippet: An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and anti-HA antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.

    Techniques Used: Activation Assay, Mutagenesis, Virus, SDS Page, Control, Staining



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    An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and <t>anti-HA</t> antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.
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    An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and <t>anti-HA</t> antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.
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    An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and <t>anti-HA</t> antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.
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    Image Search Results


    An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and anti-HA antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.

    Journal: The Plant Cell

    Article Title: The NRC0 gene cluster of sensor and helper NLR immune receptors is functionally conserved across asterid plants

    doi: 10.1093/plcell/koae154

    Figure Lengend Snippet: An autoactive NRC0-dependent sensor leads to the formation of an NRC0 higher-order complex in N. benthamiana . A) A schematic representation of helper NRC activation by sensor NLRs. B) The detection of an activated NRC0 complex in BN-PAGE. Each Agrobacterium strain carrying a wild-type SlNRC0 sensor (SlNRC0-Sa), a SlNRC0-Sa MHD mutant (SlNRC0-Sa DV ), a MADA motif mutant of SlNRC0 (SlNRC0 EEE ), Potato virus X CP, a wild-type Rx (Rx), or a MADA motif mutant of NRC2 (NRC2 EEE ) was inoculated to the leaves of an N. benthamiana nrc2 nrc3 nrc4 mutant line. Total proteins were extracted from the inoculated leaves at 3 d after agroinfiltration. Extracts were run on native and SDS–PAGE gels and immunoblotted with anti-FLAG, anti-V5, and anti-HA antibodies, respectively. Loading control was visualized with Ponceau-S staining. The higher-order complex of activated NRC0 was detected in 3 independent experiments.

    Article Snippet: Immunoblotting was performed using the antibodies, anti-HA (3F10) HRP (Roche), anti-V5 (V2260) HRP (Roche), and anti-FLAG (M2) (Sigma), in a 1:5,000 dilution ratio.

    Techniques: Activation Assay, Mutagenesis, Virus, SDS Page, Control, Staining